The opposition Conservative Party has demanded Channel 4 cancel plans to air the program on June 6 because it may distress Diana's sons, Princes William and Harry.
Channel 4 said it had "carefully and sensitively selected" the images and that in a photo showing the car's occupants, the victims had been blacked out. It said that image had previously been published in the British press and that reports about the film's content were inaccurate.
"These photographs are an important and accurate eyewitness record of how events unfolded after the crash," Channel 4 said in a statement. "We acknowledge there is great public sensitivity surrounding pictures of the victims and these have not been included."
Channel 4 said the film was produced by its history department and there was "genuine public interest" in how the events leading to Diana's death had unfolded.
The Conservative Party's culture critic, Hugo Swire, said the program was a new violation of Diana's privacy.
"This kind of coverage must be deeply distressing to Princes William and Harry," Swire said. "It is difficult to see who will be served from broadcasting such sensational and private material."
A spokesman for William and Harry would not comment specifically on the documentary but said the princes had made their position "very clear a number of times in the past."
"Diana's memory should be left as it is. This kind of thing is distasteful to her family and friends," the spokesman said.
A French investigation ruled that Paul was drunk and lost control of the car while trying to evade photographers. The British investigation concluded that Diana was not pregnant or about to marry Fayed, and that the crash was caused by Paul, who was drunk and speeding.
A full jury inquest into the accident - which is held under British law when someone dies unexpectedly, violently or of unknown causes - is expected to start in October.
